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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

《化学科学与工程前沿（英文）》 2012年第6卷第2期页码 203-209 doi: 10.1007/s11705-012-1277-2

摘要： Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: , and , including yeasts and . Its antibacterial activity was investigated on gram-negative bacteria . These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec.Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.

关键词： recombinant destabilase-lysozyme (rec.Dest-Lys) antimicrobial activity antifungal activity medicinal leech heated-up derivative of rec.Dest-Lys (T-rec.Dest-Lys)

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Thermal and energy analysis of a Chinese kang

Zhi ZHUANG, Yuguo LI, Xudong YANG, Bin CHEN, Jiaping LIU,

《能源前沿（英文）》 2010年第4卷第1期页码 84-92 doi: 10.1007/s11708-010-0009-x

摘要： About 67million Chinese kangs are still used by about 175million people in China today. The kang utilizes biomass burning for space heating and hence reduces the use of commercial energy. However, the existing design of kangs is largely based on the accumulated experience of craftsmen, for lack of scientific studies and engineering design guidelines. Poor construction of kangs also leads to serious indoor air pollution. In this paper, a macroscopic smoke flow and heat transfer model of an elevated kang is integrated in a widely-used building energy analysis software in China–DeST, and the integrated software can be used for the thermal performance analysis of kang-integrated houses. A typical house with kangs in various villages of northern China is chosen as a case study. The annual performance of the kang under different insulations of building envelope and climatic conditions are discussed. Based on the simulation results, it is found that the thermal comfort requirement of the outside surface of the upper kang plate can be met by a proper construction of the kang and selection of the appropriate firing pattern. The better the insulation of the building envelope is or the better the room air tightness is, the greater the indoor air temperature rise and the greater the building heating load contribution of the kang. In the eight selected cities in northern China, the use of kang can meet 50%–80% of the house heating load.

关键词： Chinese kang elevated kang rural house thermal modeling building energy efficiency DeST